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ip 3 rs  (Bio-Rad)


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    Bio-Rad ip 3 rs
    Ip 3 Rs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing <t>type</t> <t>3</t> IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.
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    Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing <t>type</t> <t>3</t> IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.
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    Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing <t>type</t> <t>3</t> IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.
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    Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing <t>type</t> <t>3</t> IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.
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    Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing <t>type</t> <t>3</t> IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.
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    Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing <t>type</t> <t>3</t> IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.
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    Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing <t>type</t> <t>3</t> IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.
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    Image Search Results


    Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing type 3 IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.

    Journal: Science signaling

    Article Title: All three IP 3 receptor isoforms generate Ca 2+ puffs that display similar characteristics *

    doi: 10.1126/scisignal.aau0344

    Figure Lengend Snippet: Distribution of puff sites throughout the interior of WT cells and cells expressing individual IP3R isoforms, visualized by lattice light-sheet microscopy. (A,B) The images illustrate a single, diagonal light-sheet section through a cell expressing type 3 IP3Rs. The plasma membrane is depicted in red, and local Ca2+ puffs (ΔF/F0) in green. Images of puffs arising at different times and locations are superimposed on the membrane image. Representative examples are shown of puffs arising immediately adjacent to the plasma membrane (A) and deep in the interior of the same cell (B); scale bar is 5 μm. (C) Bar graph summarizing the percentages of puffs arising near the cell edge or deeper (>2 μm) within the cell interior in WT (n = 42), IP3R1 (8), IP3R2 (12), and IP3R3 (9) cells.

    Article Snippet: Antibodies against type 1 and 2 IP 3 Rs were from Pocono Rabbit Farms and Laboratories, and antibodies against type 3 IP 3 Rs were from BD Transduction Laboratories.

    Techniques: Expressing, Microscopy